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Craig Glover, an organiser, said: "It's been absolutely fantastic. Everyone has really enjoyed themselves - it was a really good atmosphere. We did a simple rock groove and played for at least 10 minutes."
The charity event, called Stick It To MS, has so far raised £20,000 for the Multiple Sclerosis Society, although organisers hope to reach £50,000.
Drummers as young as five and as old as 50 travelled from across the UK to take part in the record bid, believed to be the UK's first attempt.
A number of special guest drummers joined in, including Slade's Don Powell and Cold Feet and Coronation Street actor John Thomson.
Mr Glover, 41, from Marston Green, Birmingham, added: "We have video evidence and certificates, which we will now send off to get verified by Guinness World Records."