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The event was organised by the United Nations children's agency UNICEF, which gave each participant a wristband with his or her number in the line and T-shirt certifying participation in the event.
"The latrine was of the same design as we use in third world countries -- a dry latrine -- and we formed the longest queue this morning," UNICEF spokesman Benoit Melebeck said.
"The Guinness Book of Records told us we needed to get at least 500 people in the queue to get the record," he said.
Melebeck said the event was to raise public awareness of the need and eventually funds for more pumps, wells, latrines and hygiene education for children in third world countries.
"We have 5,000 children dying every day from the lack of clean water, sanitation and hygiene education -- this means one every 20 seconds," he said.
Melebeck said none of the 756 people actually used the latrine, which was a fake.